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Int J Clin Exp Med. 2015 Jun 15;8(6):9229-38. eCollection 2015.

Expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths.

Author information

1
Department of Emergency, General Hospital of Pingmei Shenma Medical Group Pingdingshan, Henan, China.
2
Department of Orthopaedics, The Fifth Hospital of Wuhan, The Second Hospital Affiliated of Jianghan University Wuhan, Hubei, China.
3
Department of Pain Treatment, The First Affiliated Hospital of Xinjiang Medical University Urumuqi, Xinjiang, China.
4
Department of Traumatology, Xinjiang Production and Construction Corps Urumuqi, Xinjiang, China.
5
Department of Orthopaedics, The First Affiliated Hospital of Xinjiang Medical University Urumuqi, Xinjiang, China.
6
Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai, China.

Abstract

BACKGROUND:

It has been studied that the distribution of bone morphogenetic protein 2 is regular under bone defect situation.

OBJECTIVE:

To observe the expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths.

METHODS:

Forty-eight New Zealand rabbits were divided into two groups randomly. 0.5 cm bone defect and 3.0 cm bone defect were made by wire saw at the middle part of radius bone after anaesthesia.

RESULTS AND CONCLUSIONS:

Western blot results showed that in the 0.5 cm bone defect group, the expression of bone morphogenetic protein 2 of the tissues in the bone defect site was increased gradually at 1, 3, 4 weeks after operation, and the expression in each defect group was increased when compared with that immediately after injury (P<0.05). In the 3.0 cm bone defect group, the expression of bone morphogenetic protein 2 of tissues in bone defect site was increased gradually and reached to its peak at 3 weeks after the operation (P<0.05). The peak value in the 3.0 cm bone defect group was significantly higher than that in 0.5 cm bone defect group (P<0.05). The peak value was maintained in high level. The comparison of bone callus formation showed that the bone callus formation of 3.0 cm bone defect group was less than that of the 0.5 cm bone defect group at 3 and 4 weeks after operation (P<0.05). The results indicate that expression of the bone morphogenetic protein 2 in 3.0 cm bone defect site is increased significantly, but the expression level cannot make the bone defect heal itself.

KEYWORDS:

Tissue construction; bone defect; bone healing; bone length; bone tissue construction; rabbit

PMID:
26309580
PMCID:
PMC4537957

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