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J Cell Biol. 2015 Aug 31;210(5):851-64. doi: 10.1083/jcb.201412074. Epub 2015 Aug 24.

Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation.

Author information

1
Department of Pathology, The University of New Mexico School of Medicine, Albuquerque, NM 87131.
2
Department of Pathology, The University of New Mexico School of Medicine, Albuquerque, NM 87131 Department of Molecular Genetics and Microbiology, The University of New Mexico School of Medicine, Albuquerque, NM 87131 Cancer Research and Treatment Center, The University of New Mexico, Albuquerque, NM 87131.
3
Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6525 GA Nijmegen, Netherlands.
4
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037.
5
Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037 Laboratory for Allergic Disease, RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Tsurumi-ku, Yokohama 230-0045, Japan.
6
Department of Pathology, The University of New Mexico School of Medicine, Albuquerque, NM 87131 Cancer Research and Treatment Center, The University of New Mexico, Albuquerque, NM 87131 dlidke@salud.unm.edu.

Abstract

Mast cells (MCs) produce soluble mediators such as histamine and prostaglandins that are known to influence dendritic cell (DC) function by stimulating maturation and antigen processing. Whether direct cell-cell interactions are important in modulating MC/DC function is unclear. In this paper, we show that direct contact between MCs and DCs occurs and plays an important role in modulating the immune response. Activation of MCs through FcεRI cross-linking triggers the formation of stable cell-cell interactions with immature DCs that are reminiscent of the immunological synapse. Direct cellular contact differentially regulates the secreted cytokine profile, indicating that MC modulation of DC populations is influenced by the nature of their interaction. Synapse formation requires integrin engagement and facilitates the transfer of internalized MC-specific antigen from MCs to DCs. The transferred material is ultimately processed and presented by DCs and can activate T cells. The physiological outcomes of the MC-DC synapse suggest a new role for intercellular crosstalk in defining the immune response.

PMID:
26304724
PMCID:
PMC4555818
DOI:
10.1083/jcb.201412074
[Indexed for MEDLINE]
Free PMC Article

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