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Nat Struct Mol Biol. 2015 Sep;22(9):712-9. doi: 10.1038/nsmb.3075. Epub 2015 Aug 24.

Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.
2
Department of Integrative Structural and Computational Biology, Scripps Research Institute, La Jolla, California, USA.
3
California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, California, USA.

Abstract

Substrates are targeted for proteasomal degradation through the attachment of ubiquitin chains that need to be removed by proteasomal deubiquitinases before substrate processing. In budding yeast, the deubiquitinase Ubp6 trims ubiquitin chains and affects substrate processing by the proteasome, but the underlying mechanisms and the location of Ubp6 within the holoenzyme have been elusive. Here we show that Ubp6 activity strongly responds to interactions with the base ATPase and the conformational state of the proteasome. Electron microscopy analyses reveal that ubiquitin-bound Ubp6 contacts the N ring and AAA+ ring of the ATPase hexamer and is in proximity to the deubiquitinase Rpn11. Ubiquitin-bound Ubp6 inhibits substrate deubiquitination by Rpn11, stabilizes the substrate-engaged conformation of the proteasome and allosterically interferes with the engagement of a subsequent substrate. Ubp6 may thus act as a ubiquitin-dependent 'timer' to coordinate individual processing steps at the proteasome and modulate substrate degradation.

PMID:
26301997
PMCID:
PMC4560640
DOI:
10.1038/nsmb.3075
[Indexed for MEDLINE]
Free PMC Article
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