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Neuron. 2015 Sep 2;87(5):989-98. doi: 10.1016/j.neuron.2015.07.011. Epub 2015 Aug 20.

Clonally Related Forebrain Interneurons Disperse Broadly across Both Functional Areas and Structural Boundaries.

Author information

1
NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA.
2
Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
3
Departments of Genetics and Ophthalmology and HHMI, Harvard Medical School, Boston, MA 02115, USA.
4
NYU Neuroscience Institute, NYU Langone Medical Center, New York, NY 10016, USA. Electronic address: fisheg01@nyumc.org.

Abstract

The medial ganglionic eminence (MGE) gives rise to the majority of mouse forebrain interneurons. Here, we examine the lineage relationship among MGE-derived interneurons using a replication-defective retroviral library containing a highly diverse set of DNA barcodes. Recovering the barcodes from the mature progeny of infected progenitor cells enabled us to unambiguously determine their respective lineal relationship. We found that clonal dispersion occurs across large areas of the brain and is not restricted by anatomical divisions. As such, sibling interneurons can populate the cortex, hippocampus striatum, and globus pallidus. The majority of interneurons appeared to be generated from asymmetric divisions of MGE progenitor cells, followed by symmetric divisions within the subventricular zone. Altogether, our findings uncover that lineage relationships do not appear to determine interneuron allocation to particular regions. As such, it is likely that clonally related interneurons have considerable flexibility as to the particular forebrain circuits to which they can contribute.

PMID:
26299473
PMCID:
PMC4560602
DOI:
10.1016/j.neuron.2015.07.011
[Indexed for MEDLINE]
Free PMC Article

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