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Injury. 2015 Nov;46(11):2146-55. doi: 10.1016/j.injury.2015.08.017. Epub 2015 Aug 13.

Neuroprotective effects of Ganoderma lucidum polysaccharides against traumatic spinal cord injury in rats.

Author information

1
Department of Neurosurgery, Turgut Ozal University, Emek, Ankara, Turkey.
2
Department of Neurosurgery, Ministry of Health, Kirikkale Yuksek Ihtisas State Hospital, Kirikkale, Turkey.
3
International Centre for Hydrogen Energy Technologies (UNIDO-ICHET), Cevizlibag, Zeytinburnu 34015, Istanbul, Turkey.
4
Department of Neurosurgery, Ministry of Health, Fatih Sultan Mehmet Education and Research Hospital, Istanbul, Turkey. Electronic address: boragurer@gmail.com.
5
Department of Biochemistry, Kirikkale University, Kirikkale, Turkey.
6
Department of Pathology, Ministry of Health, Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey.
7
Department of Anatomy, Hacettepe University, Ankara, Turkey.
8
Department of Emergency Medicine, Bulent Ecevit University, Zonguldak, Turkey.

Abstract

INTRODUCTION:

Ganoderma lucidum (G. lucidum) is a mushroom belonging to the polyporaceae family of Basidiomycota and has widely been used as a traditional medicine for thousands of years. G. lucidum has never been studied in traumatic spinal cord injury. The aim of this study is to investigate whether G. lucidum polysaccharides (GLPS) can protect the spinal cord after experimental spinal cord injury.

MATERIALS AND METHODS:

Rats were randomized into five groups of eight animals each: control, sham, trauma, GLPS, and methylprednisolone. In the control group, no surgical intervention was performed. In the sham group, only a laminectomy was performed. In all the other groups, the spinal cord trauma model was created by the occlusion of the spinal cord with an aneurysm clip. In the spinal cord tissue, caspase-3 activity, tumour necrosis factor-alpha levels, myeloperoxidase activity, malondialdehyde levels, nitric oxide levels, and superoxide dismutase levels were analysed. Histopathological and ultrastructural evaluations were also performed. Neurological evaluation was performed using the Basso, Beattie, and Bresnahan locomotor scale and the inclined-plane test.

RESULTS:

After traumatic spinal cord injury, increases in caspase-3 activity, tumour necrosis factor-alpha levels, myeloperoxidase activity, malondialdehyde levels, and nitric oxide levels were detected. After the administration of GLPS, decreases were observed in tissue caspase-3 activity, tumour necrosis factor-alpha levels, myeloperoxidase activity, malondialdehyde levels, and nitric oxide levels. Furthermore, GLPS treatment showed improved results in histopathological scores, ultrastructural scores, and functional tests.

CONCLUSIONS:

Biochemical, histopathological, and ultrastructural analyses and functional tests reveal that GLPS exhibits meaningful neuroprotective effects against spinal cord injury.

KEYWORDS:

Ganoderma lucidum; Methylprednisolone; Neuroprotection; Polysaccharides; Spinal cord injury; Trauma

PMID:
26298021
DOI:
10.1016/j.injury.2015.08.017
[Indexed for MEDLINE]

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