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Biomed Pharmacother. 2015 Oct;75:129-36. doi: 10.1016/j.biopha.2015.07.036. Epub 2015 Aug 19.

miR-125a-5p inhibits cell proliferation and induces apoptosis in colon cancer via targeting BCL2, BCL2L12 and MCL1.

Author information

1
Department of General Surgery, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China.
2
Department of Pathology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng St. Nangang, Harbin 150006, Heilongjiang, China.
3
Department of Surgical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China.
4
Department of Surgical Oncology, The Fourth Affiliated Hospital of Harbin Medical University, 91 Songhuajiang St. Nangang, Harbin 150006, Heilongjiang, China. Electronic address: qifanzhang2015@163.com.

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that function as regulators of gene expression. MiR-125 is a family of miRNAs that have been shown to be involved in various cancer types. In this study, for the first time, we showed that miR-125a-5p was specifically down-regulated in both colon cancer tissue and colon cancer cell lines. The tumor suppressor role of miR-125a-5p in colon cancer was supported by the observation that overexpression of miR-125a-5p inhibited cell proliferation and induced cell apoptosis in colon cancer cells. We also confirmed that in colon cancer cells, anti-apoptotic genes BCL2, BCL2L12 and Mcl-1 were direct targets of miR-125a-5p, and they were down-regulated by miR-125a-5p overexpression. Furthermore, restoration of BCL2, BCL2L12 and Mcl-1 expression in colon cancer cells could partially reverse the cell proliferation inhibition and apoptosis stimulation caused by miR-125a-5p overexpression, indicating that miR-125a-5p inhibits cell proliferation and induces apoptosis in colon cancer cells via targeting BCL2, BCL2L12 and Mcl-1.

KEYWORDS:

BCL2; BCL2L12; Colon cancer; Mcl-1; miR-125a-5p

PMID:
26297542
DOI:
10.1016/j.biopha.2015.07.036
[Indexed for MEDLINE]

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