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Neuropharmacology. 2016 Jul;106:102-15. doi: 10.1016/j.neuropharm.2015.08.025. Epub 2015 Aug 19.

Identification of histaminergic neurons through histamine 3 receptor-mediated autoinhibition.

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Department of Neurophysiology, Heinrich-Heine-Universität, Medical Faculty, D-40225 Düsseldorf, Germany.
Institute for Pharmacology and Clinical Pharmacology, Heinrich-Heine-Universität, Medical Faculty, D-40225 Düsseldorf, Germany.
Department of Neurophysiology, Heinrich-Heine-Universität, Medical Faculty, D-40225 Düsseldorf, Germany. Electronic address:


Using a reporter mouse model with expression of the tomato fluorescent protein under the dopamine transporter promoter (Tmt-DAT) we discovered a new group of neurons in the histaminergic tuberomamillary nucleus (TMN), which, in contrast to tuberoinfundibular dopaminergic neurons of the dorsomedial arcuate nucleus, do not express tyrosine hydroxylase but can synthesize and store dopamine. Tmt-DAT neurons located within TMN share electrophysiological properties with histaminergic neurons: spontaneous firing at a membrane potential around -50 mV and presence of hyperpolarization-activated cyclic nucleotide-gated ion channels. Histamine (30 μM) depolarizes and excites Tmt-DAT neurons through H1R activation but inhibits histaminergic neurons through H3R activation thus allowing a pharmacological identification of the different neurons. Single-cell RT-PCR revealed that all histaminergic neurons expressing histidine decarboxylase (HDC) also express H3R. This includes neurons retrogradely traced from the striatum whose inhibition by a selective H3R agonist was indistinguishable from the whole population. Prolonged depolarization reduces the autoinhibition. The potency of histamine at H3R depends on membrane potential and on extracellular and intracellular calcium. Autoinhibition can be impaired by preincubation with capsaicin, a ligand of the calcium-permeable TRPV1 channel or by blockade of Ca(2+)-ATPase with thapsigargin. The pharmacology of autoinhibition is revisited and physiological conditions for its functionality are determined. Usage of reporter mouse models for the safe identification of aminergic neurons under pathophysiological conditions is recommended. This article is part of the Special Issue entitled 'Histamine Receptors'.


Aminergic neurons; Autoinhibition; H(1) receptor; H(3) receptor; Patch-clamp; Retrograde tracing; Single cell RT-PCR

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