Format

Send to

Choose Destination
Int J Biol Macromol. 2015 Nov;81:461-6. doi: 10.1016/j.ijbiomac.2015.08.023. Epub 2015 Aug 19.

Time-dependent viscometry study of endoglucanase action on xyloglucan: A real-time approach.

Author information

1
BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil.
2
BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil; Laboratory of Plant Carbohydrates, Department of Biochemistry, Federal University of Paraná, 81531-990 Curitiba, PR, Brazil.
3
Laboratory of Plant Carbohydrates, Department of Biochemistry, Federal University of Paraná, 81531-990 Curitiba, PR, Brazil.
4
BioPol, Chemistry Department, Federal University of Paraná, P.O. Box 19032, 81531-980 Curitiba, PR, Brazil. Electronic address: rilton@ufpr.br.

Abstract

Hydrolysis of xyloglucan from Tamarindus indica and Hymenaea courbaril seeds with endoglucanase (EGII), which randomly breaks the (1→4)-linked β-glycosidic bonds of the polymer chain, was monitored in real time using time-dependent viscometry analysis (TDV). For both samples there was a decrease in the intrinsic viscosity ([η]), viscosity average molar mass (Mv), radius de gyration (Rg) and persistence length (Lp) immediately after the addition of the enzyme. It was observed the formation of oligosaccharides and oligomers composed of ∼2 units, up to 140min. Galactose-containing side chains two positions away from the non-substituted glucose, modulated the action of EGII, and the complete hydrolysis of the XG oligomers occurred after 24h. The results demonstrate for the first time the real-time degradation of xyloglucan as well the macromolecular and oligosaccharide composition during the EGII hydrolysis process.

KEYWORDS:

Endoglucanase; Time-dependent viscometry; Xyloglucan

PMID:
26297307
DOI:
10.1016/j.ijbiomac.2015.08.023
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center