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Plant Cell Environ. 2016 Feb;39(2):393-415. doi: 10.1111/pce.12624. Epub 2015 Oct 27.

Integrated multi-omics analysis supports role of lysophosphatidylcholine and related glycerophospholipids in the Lotus japonicus-Glomus intraradices mycorrhizal symbiosis.

Author information

1
Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, University of Cologne, Zuelpicher Str. 47b, D-50674, Cologne, Germany.
2
Department of Plant Pathology, The Ohio State University, Kottman Hall, 2021 Coffey Road, Columbus, OH, 43210, USA.
3
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053, Regensburg, Germany.
4
Bayer CropScience AG, Alfred-Nobel-Straße 50, D-40789, Monheim am Rhein, Germany.
5
Plant Molecular Physiology and Biotechnology, Heinrich Heine University, D-40225, Düsseldorf, Germany.

Abstract

Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus, we performed an integrated multi-omics analysis with a focus on plant and fungal phospholipid (PL) metabolism and biological significance of lysophosphatidylcholine (LPC). Our results support the role of LPC as a bioactive compound eliciting cellular and molecular response mechanisms in Lotus. Evidence is provided for large interspecific chemical diversity of LPC species among mycorrhizae with related AMF species. Lipid, gene expression and elemental profiling emphasize the Lotus-Glomus intraradices interaction as distinct from other arbuscular mycorrhizal (AM) interactions. In G. intraradices, genes involved in fatty acid (FA) elongation and biosynthesis of unsaturated FAs were enhanced, while in Lotus, FA synthesis genes were up-regulated during AMS. Furthermore, FAS protein localization to mitochondria suggests FA biosynthesis and elongation may also occur in AMF. Our results suggest the existence of interspecific partitioning of PL resources for generation of LPC and novel candidate bioactive PLs in the Lotus-G. intraradices symbiosis. Moreover, the data advocate research with phylogenetically diverse Glomeromycota species for a broader understanding of the molecular underpinnings of AMS.

KEYWORDS:

Glomeromycota; Glomus intraradices (syn: Rhizophagus irregularis); arbuscular mycorrhiza; fatty acid synthesis; ionomics; lipidomics; phospholipids; transcriptomics

PMID:
26297195
DOI:
10.1111/pce.12624
[Indexed for MEDLINE]
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