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Mol Cell. 2015 Aug 20;59(4):588-602. doi: 10.1016/j.molcel.2015.07.020.

Condensin- and Replication-Mediated Bacterial Chromosome Folding and Origin Condensation Revealed by Hi-C and Super-resolution Imaging.

Author information

1
Institut Pasteur, Department of Genomes and Genetics, Group Spatial Regulation of Genomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France.
2
Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France.
3
Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, NE2 4AX Newcastle Upon Tyne, UK.
4
Laboratoire de Physique Théorique de la Matière Condensée, CNRS UMR 7600, Université Pierre et Marie Curie, Sorbonne Universités, 75005 Paris, France.
5
Institut Pasteur, Department of Genomes and Genetics, Group Spatial Regulation of Genomes, 75015 Paris, France; CNRS, UMR 3525, 75015 Paris, France. Electronic address: romain.koszul@pasteur.fr.
6
Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 Rue de Navacelles, 34090 Montpellier, France. Electronic address: marcelo.nollmann@cbs.cnrs.fr.

Abstract

Chromosomes of a broad range of species, from bacteria to mammals, are structured by large topological domains whose precise functional roles and regulatory mechanisms remain elusive. Here, we combine super-resolution microscopies and chromosome-capture technologies to unravel the higher-order organization of the Bacillus subtilis chromosome and its dynamic rearrangements during the cell cycle. We decipher the fine 3D architecture of the origin domain, revealing folding motifs regulated by condensin-like complexes. This organization, along with global folding throughout the genome, is present before replication, disrupted by active DNA replication, and re-established thereafter. Single-cell analysis revealed a strict correspondence between sub-cellular localization of origin domains and their condensation state. Our results suggest that the precise 3D folding pattern of the origin domain plays a role in the regulation of replication initiation, chromosome organization, and DNA segregation.

KEYWORDS:

DNA replication; bacterial mitosis; chromosome conformation capture; chromosome segregation; chromosome structure and organization; condensins; super-resolution microscopy

PMID:
26295962
DOI:
10.1016/j.molcel.2015.07.020
[Indexed for MEDLINE]
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