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J Hematol Oncol. 2015 Aug 21;8:100. doi: 10.1186/s13045-015-0197-2.

Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion.

Author information

1
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. ping.chengcheng@163.com.
2
Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. ping.chengcheng@163.com.
3
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. qiuhuiying@suda.edu.cn.
4
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. shirley-1214@163.com.
5
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. daihaiping8@126.com.
6
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. changgengruan@hotmail.com.
7
Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. changgengruan@hotmail.com.
8
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. Stefan.Ehrentraut@dsmz.de.
9
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. hdr@dsmz.de.
10
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. rml@dsmz.de.
11
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. chensuning@suda.edu.cn.
12
Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. chensuning@suda.edu.cn.

Abstract

Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384.

PMID:
26293203
PMCID:
PMC4546145
DOI:
10.1186/s13045-015-0197-2
[Indexed for MEDLINE]
Free PMC Article

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