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J Clin Microbiol. 2015 Nov;53(11):3621-3. doi: 10.1128/JCM.01404-15. Epub 2015 Aug 19.

One-Step Multiplex PCR Assay for Differentiating Proposed New Species "Clostridium neonatale" from Closely Related Species.

Author information

1
EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, DHU Risques et Grossesse, Sorbonne Paris Cité, Paris, France.
2
Institut Pasteur, Unité des Bactéries Anaérobies et Toxines, Paris, France.
3
Andromas SAS, Paris, France.
4
EA4065 Ecosystème Intestinal, Probiotiques, Antibiotiques, DHU Risques et Grossesse, Sorbonne Paris Cité, Paris, France julio.aires@parisdescartes.fr.

Abstract

"Clostridium neonatale" sp. nov., previously involved in an outbreak of neonatal necrotizing enterocolitis, was recently proposed as a new species of the Clostridium genus sensu stricto. We developed a one-step multiplex colony PCR for C. neonatale identification and investigated C. neonatale intestinal colonization frequency in healthy preterm neonates.

PMID:
26292306
PMCID:
PMC4609713
DOI:
10.1128/JCM.01404-15
[Indexed for MEDLINE]
Free PMC Article

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