A novel electrochemical immunosensor for the quantitative detection of 5-hydroxymethylcytosine in genomic DNA of breast cancer tissue

Chem Commun (Camb). 2015 Oct 7;51(78):14671-3. doi: 10.1039/c5cc05921f.

Abstract

A novel electrochemical immunosensor was fabricated for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA based on anti-5-hmC antibody, biotin functionalized phos-tag and avidin functionalized alkaline phosphatase. It is demonstrated that the levels of 5-hmC are dramatically reduced in human breast cancer tissue compared with those in normal tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Methylcytosine / analogs & derivatives
  • Biosensing Techniques*
  • Breast Neoplasms / genetics*
  • Cytosine / analogs & derivatives*
  • Cytosine / analysis
  • DNA, Neoplasm / chemistry*
  • Female
  • Humans

Substances

  • DNA, Neoplasm
  • 5-hydroxymethylcytosine
  • 5-Methylcytosine
  • Cytosine