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Cell Biol Int. 2015 Dec;39(12):1418-24. doi: 10.1002/cbin.10537. Epub 2015 Oct 13.

Protection of seven dibenzocyclooctadiene lignans from Schisandra chinensis against serum and glucose deprivation injury in SH-SY5Y cells.

E Q1, Tang M2,3, Zhang X2,3, Shi Y2,3, Wang D2,3, Gu Y2,3, Li S2,3, Liang X2,3,4, Wang Z2,3,5, Wang C2,3.

Author information

1
Department of Pathology, School of Medicine, Nantong University, Nantong, 226001, P. R. China.
2
Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
3
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, P. R. China.
4
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, 116023, P. R. China.
5
Department of Pharmacology, University of California, Irvine, California, 92697, USA.

Abstract

Dibenzocyclooctadiene lignans, the major active components of fruit of Schisandra chinensis (Turcz.) Baill., have been found to have activities that could prevent prostate and thyroid cancer, hepatotoxicity, oxidative stress-induced cerebral injury, etc. This study was conducted to evaluate the effects of seven dibenzocyclooctadiene lignans of Schisandra chinensis and explore the possible mechanisms in the human neuroblastoma SH-SY5Y cells exposed on serum and glucose deprivation (SGD) injury. The structure-activity relationships were also analyzed. Cell viability and lactate dehydrogenase (LDH) release were determined to evaluate cell injury. Inflammation and apoptosis-related protein levels were detected to elucidate the possible mechanisms. Schisantherin A, schizandrin C, and schizandrol B were found to have stronger protective effects than schizandrin A, schizandrin B, and schisanhenol in SH-SY5Y cells against SGD injury. Moreover, the protective effects of these lignans were possibly exhibited by regulating inflammation and apoptosis-related proteins in SH-SY5Y cells after SGD injury, supporting their beneficial effects for the prevention of cell injury in the pathogenesis of the central nervous system diseases, including ischemia stroke. The number and position of hydroxyl group and methylenedioxy in these lignans may be required for their effects.

KEYWORDS:

Schisandra chinensis; apoptosis; dibenzocyclooctadiene lignans; inflammation; neuroprotection

PMID:
26289388
DOI:
10.1002/cbin.10537
[Indexed for MEDLINE]

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