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Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07597.

GLO-Roots: an imaging platform enabling multidimensional characterization of soil-grown root systems.

Author information

1
Department of Plant Biology, Carnegie Institution for Science, Stanford, United States.
2
PhytoSystems, University of Liège, Liège, Belgium.
3
Department of Biology, Stanford University, Stanford, United States.
4
Department of Plant Biology, University of California, Davis, Davis, United States.
5
Department of Genetics, Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, United States.
6
Western Regional Research Center, United States Department of Agriculture, Albany, United States.
7
Department of Energy, Department of Energy Joint Genome Institute, Walnut Creek, United States.

Abstract

Root systems develop different root types that individually sense cues from their local environment and integrate this information with systemic signals. This complex multi-dimensional amalgam of inputs enables continuous adjustment of root growth rates, direction, and metabolic activity that define a dynamic physical network. Current methods for analyzing root biology balance physiological relevance with imaging capability. To bridge this divide, we developed an integrated-imaging system called Growth and Luminescence Observatory for Roots (GLO-Roots) that uses luminescence-based reporters to enable studies of root architecture and gene expression patterns in soil-grown, light-shielded roots. We have developed image analysis algorithms that allow the spatial integration of soil properties, gene expression, and root system architecture traits. We propose GLO-Roots as a system that has great utility in presenting environmental stimuli to roots in ways that evoke natural adaptive responses and in providing tools for studying the multi-dimensional nature of such processes.

KEYWORDS:

Brachypodium distachyon; Lycopersicon esculentum; arabidopsis; imaging systems; plant biology

PMID:
26287479
PMCID:
PMC4589753
DOI:
10.7554/eLife.07597
[Indexed for MEDLINE]
Free PMC Article

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