Format

Send to

Choose Destination
Nat Rev Mol Cell Biol. 2015 Sep;16(9):533-44. doi: 10.1038/nrm4032. Epub 2015 Aug 19.

Specificity and nonspecificity in RNA-protein interactions.

Author information

1
Center for RNA Molecular Biology, Case Western Reserve University 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.
2
Department of Biochemistry, Case Western Reserve University 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.
3
Department of Physics, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.

Abstract

To fully understand the regulation of gene expression, it is critical to quantitatively define whether and how RNA-binding proteins (RBPs) discriminate between alternative binding sites in RNAs. Here, we describe new methods that measure protein binding to large numbers of RNA variants, and ways to analyse and interpret data obtained by these approaches, including affinity distributions and free energy landscapes. We discuss how the new methodologies and the associated concepts enable the development of inclusive, quantitative models for RNA-protein interactions that transcend the traditional binary classification of RBPs as either specific or nonspecific.

PMID:
26285679
PMCID:
PMC4744649
DOI:
10.1038/nrm4032
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center