(a) FPKM values for BMP receptor and Smad genes expressed by GFP+ and GFP− LSK SLAM sorted HSCs from E14 FL and adult BM. ND, not detected. (b–d) Gene list enrichment analysis on genes with more than two fold difference in expression level between GFP+ and GFP− LSK SLAM sorted cells from FL and BM using Enrichr web-application (threshold false discovery rate (FDR)=0.01). GFP+ high genes are enriched in (b) BMPR2 gene targets (FDR<1.0e−13; Gene Expression Omnibus (GEO) Kinase perturbation gene sets). Gene lists available on request. (c) Also, overrepresented in GFP+ high genes are genes involved in homoeostasis (FDR=9.8e−4; Mouse Genome Informatics (MGI) mammalian phenotype term MP0001764), metabolism (FDR=5.4e−4; MGI mammalian phenotype term MP0005266), MYC (FDR=2.7e−4; TRANSFAC and JASPAR Position Weight Matrix (PWM) genesets), and STAT5B (FDR=2.7e−4; TRANSFAC and JASPAR PWM gene sets); (d) GFP− high genes are enriched for NFE2 (FDR=9.6e−3; TRANSFAC and JASPAR PWM gene sets), NFKB1 (FDR=2.7e−3; TRANSFAC and JASPAR PWM gene sets), SP1 (FDR=2.7e−3; TRANSFAC and JASPAR PWM gene sets), and haematopoietic system (FDR=5.2e−7; MGI mammalian phenotype term MP0002396)-related genes.