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Stem Cell Res Ther. 2015 Aug 18;6:144. doi: 10.1186/s13287-015-0137-7.

Mesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity.

Author information

1
Orthopedic Research Lab, Aarhus University, 8000, Aarhus C, Denmark. KANG.RAN@CLIN.AU.DK.
2
Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China. KANG.RAN@CLIN.AU.DK.
3
Department of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. YAZH@BIOMED.AU.DK.
4
Department of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. SHUANG.TAN@HUM-GEN.AU.DK.
5
Shenzhen Key Laboratory for Anti-aging and Regenerative Medicine, Health Science Center, Shenzhen University, 518060, Shenzhen, China. SHUANG.TAN@HUM-GEN.AU.DK.
6
Shenzhen Key Laboratory for Anti-aging and Regenerative Medicine, Health Science Center, Shenzhen University, 518060, Shenzhen, China. GQZHOU@SZU.EDU.CN.
7
Department of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. AAGAARD@BIOMED.AU.DK.
8
Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, 210028, China. XIELIN117@126.COM.
9
Orthopedic Research Lab, Aarhus University, 8000, Aarhus C, Denmark. CODYBUNG@RM.DK.
10
Department of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. BOLUND@BIOMED.AU.DK.
11
Department of Biomedicine, the Health Faculty, Aarhus University, 8000, Aarhus C, Denmark. ALUN@BIOMED.AU.DK.

Abstract

INTRODUCTION:

Previously, we established a simple method for deriving mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in scaffolds and nanofibers. The objective of this study is to systematically characterize the mesenchymal characteristics of the iPSC-MSCs by comparing them to bone marrow-derived MSCs (BM-MSCs).

METHODS:

Two iPSC-MSC lines (named as mRNA-iPSC-MSC-YL001 and lenti-iPSC-MSC-A001) and one BM-MSC line were used for the study. Cell proliferation, presence of mesenchymal surface markers, tri-lineage differentiation capability (osteogenesis, chondrogenesis, adipogenesis), and expression of "stemness" genes were analyzed in these MSC lines.

RESULTS:

The iPSC-MSCs were similar to BM-MSCs in terms of cell morphology (fibroblast-like) and surface antigen profile: CD29+, CD44+, CD73+, CD90+, CD105+, CD11b-, CD14-, CD31-, CD34-, CD45- and HLA-DR-. A faster proliferative capability was seen in both iPSC-MSCs lines compared to the BM-MSCs. The iPSC-MSCs showed adequate capacity of osteogenesis and chondrogenesis compared to the BM-MSCs, while less adipogenic potential was found in the iPSC-MSCs. The iPSC-MSCs and the tri-lineage differentiated cells (osteoblasts, chondrocytes, adipocytes) all lack expression of "stemness" genes: OCT4, SOX2, GDF3, CRIPTO, UTF1, DPPA4, DNMT3B, LIN28a, and SAL4.

CONCLUSIONS:

The MSCs derived from human iPSCs with our method have advanced proliferation capability and adequate osteogenic and chondrogenic properties compared to BM-MSCs. However, the iPSC-MSCs were less efficient in their adipogenicity, suggesting that further modifications should be applied to our method to derive iPSC-MSCs more closely resembling the naïve BM-MSCs if necessary.

PMID:
26282538
PMCID:
PMC4539932
DOI:
10.1186/s13287-015-0137-7
[Indexed for MEDLINE]
Free PMC Article

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