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Nat Methods. 2015 Oct;12(10):963-965. doi: 10.1038/nmeth.3542. Epub 2015 Aug 17.

ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors.

Author information

1
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
2
Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
3
Max Planck Institute for Informatics, Saarbrücken, Germany.
#
Contributed equally

Abstract

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.

PMID:
26280331
PMCID:
PMC4589892
DOI:
10.1038/nmeth.3542
[Indexed for MEDLINE]
Free PMC Article

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