Format

Send to

Choose Destination
Nat Methods. 2015 Oct;12(10):943-6. doi: 10.1038/nmeth.3541. Epub 2015 Aug 17.

EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.

Author information

1
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
2
Graduate Group in Biophysics, University of California, San Francisco, San Francisco, California, USA.
3
Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
4
Graduate Program in Biological Physics, Structure and Design, University of Washington, Seattle, Washington, USA.
5
Department of Biochemistry, University of Washington, Seattle, Washington, USA.
6
Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
7
Institute for Protein Design, Seattle, Washington, USA.
8
Department of Bioengineering, University of California, Berkeley, Berkeley, California, USA.

Abstract

Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.

PMID:
26280328
PMCID:
PMC4589481
DOI:
10.1038/nmeth.3541
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center