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Nat Commun. 2015 Aug 17;6:8055. doi: 10.1038/ncomms9055.

Cell shape dynamics during the staphylococcal cell cycle.

Author information

1
Laboratory of Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.
2
Laboratory of Bacterial Cell Surfaces and Pathogenesis, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.
3
1] Department of Chemistry, Indiana University Bloomington, Bloomington, Indiana 47405, USA [2] Department of Biology, Indiana University Bloomington, Bloomington, Indiana 47405, USA.
4
Department of Chemistry, Indiana University Bloomington, Bloomington, Indiana 47405, USA.
5
Department of Biology, Indiana University Bloomington, Bloomington, Indiana 47405, USA.

Abstract

Staphylococcus aureus is an aggressive pathogen and a model organism to study cell division in sequential orthogonal planes in spherical bacteria. However, the small size of staphylococcal cells has impaired analysis of changes in morphology during the cell cycle. Here we use super-resolution microscopy and determine that S. aureus cells are not spherical throughout the cell cycle, but elongate during specific time windows, through peptidoglycan synthesis and remodelling. Both peptidoglycan hydrolysis and turgor pressure are required during division for reshaping the flat division septum into a curved surface. In this process, the septum generates less than one hemisphere of each daughter cell, a trait we show is common to other cocci. Therefore, cell surface scars of previous divisions do not divide the cells in quadrants, generating asymmetry in the daughter cells. Our results introduce a need to reassess the models for division plane selection in cocci.

PMID:
26278781
PMCID:
PMC4557339
DOI:
10.1038/ncomms9055
[Indexed for MEDLINE]
Free PMC Article

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