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Oncol Res Treat. 2015;38(7-8):362-6. doi: 10.1159/000433499. Epub 2015 Jul 1.

GAS5 Inhibits Gastric Cancer Cell Proliferation Partly by Modulating CDK6.

Author information

1
Department of General Surgery, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, China.

Abstract

INTRODUCTION:

As it is not clear whether growth arrest-specific 5 (GAS5) inhibits gastric cancer (GC) cell proliferation by regulating cell cycle, we analyzed the effect of GAS5 on cell cycle regulation of GC cells and explored the underlying mechanism.

METHODS:

We measured GAS5 levels in GC tissues and corresponding normal tissues, and analyzed the role of GAS5 in regulation of cell proliferation and cell cycle in GC cells using CCK-8 assay and flow cytometry. We also measured the expression of P21 and CDK6 proteins after transfection of AGS and MGC-803 cells with pLJM-GAS5 and GAS5 siRNA, respectively, by western blotting.

RESULTS:

GAS5 expression was significantly lower in GC tissues relative to normal tissues, and its lower expression was correlated with larger tumor size and a more advanced clinical stage of GC. GAS5 induced growth arrest of GC cells through inhibition of G1-S phase translation. The action of GAS5 may be mediated by upregulation of P21 and suppression of CDK6.

CONCLUSION:

These data enhance our understanding of the important role that GAS5 plays in the molecular etiology of GC and suggest a potential of GAS5 as a new therapeutic target for GC treatment.

PMID:
26278580
DOI:
10.1159/000433499
[Indexed for MEDLINE]

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