Format

Send to

Choose Destination
Appl Microbiol Biotechnol. 2015 Dec;99(24):10639-54. doi: 10.1007/s00253-015-6925-2. Epub 2015 Aug 18.

The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli.

Author information

1
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. zgxiangkai@163.com.
2
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 2013207013@njau.edu.cn.
3
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 850421558@qq.com.
4
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. xmao@njau.edu.cn.
5
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 2013807115@njau.edu.cn.
6
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. 1196820085@qq.com.
7
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 1091517919@qq.com.
8
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. lucp@njau.edu.cn.
9
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. fhj@njau.edu.cn.
10
Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. daijianjun@njau.edu.cn.

Abstract

Autotransporters (ATs) are associated with pathogenesis of Avian Pathogenic Escherichia coli (APEC). The molecular characterization of APEC ATs can provide insights about their relevance to APEC pathogenesis. Here, we characterized a conventional autotransporter UpaB in APEC DE205B genome. The upaB existed in 41.9 % of 236 APEC isolates and was predominantly associated with ECOR B2 and D. Our studies showed that UpaB mediates the DE205B adhesion in DF-1 cells, and enhances autoaggregation and biofilm formation of fimbria-negative E. coli AAEC189 (MG1655Δfim) in vitro. Deletion of upaB of DE205B attenuates the virulence in duck model and early colonization in the duck lungs during APEC systemic infection. Furthermore, double and triple deletion of upaB, aatA, and aatB genes cumulatively attenuated DE205B adhesion in DF-1 cells, accompanying with decreased 50 % lethal dose (LD50) in duck model and the early colonization in the duck lungs. However, DE205BΔupaB/ΔaatA/ΔaatB might "compensate" the influence of gene deletion by upregulating the expression of fimbrial adhesin genes yqiL, yadN, and vacuolating autotransporter vat during early colonization of APEC. Finally, we demonstrated that vaccination with recombinant UpaB, AatA, and AatB proteins conferred protection against colisepticemia caused by DE205B infection in duck model.

KEYWORDS:

APEC; Autotransporter UPAB; Double/triple deletion; Pathogenicity; Vaccination

PMID:
26278540
DOI:
10.1007/s00253-015-6925-2
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center