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Colloids Surf B Biointerfaces. 2015 Nov 1;135:386-93. doi: 10.1016/j.colsurfb.2015.07.057. Epub 2015 Jul 26.

Nano-hydroxyapatite-coated metal-ceramic composite of iron-tricalcium phosphate: Improving the surface wettability, adhesion and proliferation of mesenchymal stem cells in vitro.

Author information

1
Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
2
Department of Orthopaedics, Medical University Graz, 8036 Graz, Austria; Institute for Interfacial Process Engineering and Plasma Technology IGVP, University of Stuttgart, Nobel street 12, 70569 Stuttgart, Germany.
3
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany.
4
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany; Process Analysis & Technology (PA&T), Reutlingen-University, 72762 Reutlingen, Germany.
5
Fraunhofer Institute for Manufacturing Technology and Advanced Materials IFAM, 28359 Bremen, Germany.
6
Technical Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45141 Essen, Germany.
7
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, 45117 Essen, Germany.
8
Department of Experimental Physics, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia; Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), 70569 Stuttgart, Germany. Electronic address: rsurmenev@gmail.com.

Abstract

Thin radio-frequency magnetron sputter deposited nano-hydroxyapatite (HA) films were prepared on the surface of a Fe-tricalcium phosphate (Fe-TCP) bioceramic composite, which was obtained using a conventional powder injection moulding technique. The obtained nano-hydroxyapatite coated Fe-TCP biocomposites (nano-HA-Fe-TCP) were studied with respect to their chemical and phase composition, surface morphology, water contact angle, surface free energy and hysteresis. The deposition process resulted in a homogeneous, single-phase HA coating. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells (hMSCs) was studied using biological short-term tests in vitro. The surface of the uncoated Fe-TCP bioceramic composite showed an initial cell attachment after 24h of seeding, but adhesion, proliferation and growth did not persist during 14 days of culture. However, the HA-Fe-TCP surfaces allowed cell adhesion, and proliferation during 14 days. The deposition of the nano-HA films on the Fe-TCP surface resulted in higher surface energy, improved hydrophilicity and biocompatibility compared with the surface of the uncoated Fe-TCP. Furthermore, it is suggested that an increase in the polar component of the surface energy was responsible for the enhanced cell adhesion and proliferation in the case of the nano-HA-Fe-TCP biocomposites.

KEYWORDS:

Bioceramic composite; Bioresorbable alloy; Cell adhesion; Hydroxyapatite coating; RF magnetron sputtering

PMID:
26277713
DOI:
10.1016/j.colsurfb.2015.07.057
[Indexed for MEDLINE]

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