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Photochem Photobiol. 2015 Nov;91(6):1368-75. doi: 10.1111/php.12511. Epub 2015 Sep 25.

Thermal Effects and Structural Changes of Photosynthetic Reaction Centers Characterized by Wide Frequency Band Hydrophone: Effects of Carotenoids and Terbutryn.

Author information

1
Department of Medical Physics and Informatics, University of Szeged, Szeged, Hungary.
2
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
3
Department of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.
4
Department of Optics and Quantum Electronics, University of Szeged, Szeged, Hungary.
5
Biophotonics R&D Ltd, Szeged, Hungary.
6
Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary.

Abstract

Photothermal characteristics and light-induced structural (volume) changes of carotenoid-containing and noncontaining photosynthetic reaction centers (RCs) were investigated by wide frequency band hydrophone. We found that the presence of carotenoid either does not play considerable role in the light-induced conformational movements, or these rearrangements are too slow for inducing a photoacoustic (PA) signal. The kinetic component with a few tens of microseconds, exhibited by the carotenoid-less RCs, appears to be similar to that of triplet state lifetimes, identified by other methods. The binding of terbutryn to the acceptor side is shown to affect the dynamics of the RC. Our results do not confirm large displacements or volume changes induced by the charge movements and by the charge relaxation processes in the RCs in few hundreds of microseconds time scale that accompanies the electron transfer between the primary and secondary electron acceptor quinones.

PMID:
26277346
DOI:
10.1111/php.12511
[Indexed for MEDLINE]

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