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Hepatol Int. 2015 Oct;9(4):586-93. doi: 10.1007/s12072-015-9658-5. Epub 2015 Aug 14.

Deregulation of microRNA expression in peripheral blood mononuclear cells from patients with HCV-related malignancies.

Author information

1
Department of Experimental and Clinical Medicine, Center for Systemic Manifestations of Hepatitis Viruses (MASVE), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. alepilu80@libero.it.
2
Department of Experimental and Clinical Medicine, Center for Systemic Manifestations of Hepatitis Viruses (MASVE), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. laura.gragnani@unifi.it.
3
Department of Experimental and Clinical Medicine, Center for Systemic Manifestations of Hepatitis Viruses (MASVE), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. elisa.fognani@gmail.com.
4
Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138, Bologna, Italy. elena.grandini2@unibo.it.
5
Department of Experimental and Clinical Medicine, Center for Systemic Manifestations of Hepatitis Viruses (MASVE), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. monica.monti@unifi.it.
6
Department of Experimental and Clinical Medicine, Center for Systemic Manifestations of Hepatitis Viruses (MASVE), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. cristina.stasi@unifi.it.
7
Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138, Bologna, Italy. elisabetta.loggi@unibo.it.
8
Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138, Bologna, Italy. marzia.margotti2@unibo.it.
9
Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138, Bologna, Italy. fabio.conti2@studio.unibo.it.
10
Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138, Bologna, Italy. pietro.andreone@unibo.it.
11
Department of Experimental and Clinical Medicine, Center for Systemic Manifestations of Hepatitis Viruses (MASVE), University of Florence, Largo Brambilla 3, 50134, Florence, Italy. a.zignego@dmi.unifi.it.

Abstract

BACKGROUND AND AIM:

Hepatocellular carcinoma is one of the major causes of death due to cancer worldwide, and its association with hepatitis C virus infection has been definitively established. Hepatitis C virus is also involved in the pathogenesis of non-Hodgkin's lymphoma. This is the only virus infecting humans that is able to induce two different malignancies. We analyzed the expression levels of a panel of microRNA in peripheral blood mononuclear cells of patients with hepatitis C virus-related malignancies in order to find a disease-associated deregulation and identify specific biomarkers.

METHODS:

We tested peripheral blood mononuclear cells isolated from patients with hepatocellular carcinoma, non-Hodgkin's lymphoma, hepatitis C virus without malignancies and healthy subjects for a panel of microRNA selected on the basis of previous studies. MicroRNA expression was evaluated by real-time PCR.

RESULTS:

Our results showed an upregulation of miRNA-21 and downregulation of miRNA-26b in hepatocellular carcinoma and non-Hodgkin's lymphoma patients compared to controls (p < 0.001). Deregulation of miRNA-16 and miRNA-155 was limited to lymphoma patients.

CONCLUSIONS:

This study shows that some microRNAs are differently expressed in peripheral blood mononuclear cells from hepatitis C virus patients who develop hepatocellular carcinoma or lymphoma, while others share a common behavior. Thus, analysis of the expression of microRNAs could be a noninvasive marker of hepatitis C virus-related carcinogenesis. This analysis could be a suitable tool for identifying the existence of a malignancy and also discriminating between these two hepatitis C virus-related cancers.

KEYWORDS:

Biomarker; Hepatitis C virus; Hepatocellular carcinoma; Non-Hodgkin’s lymphoma; microRNA

PMID:
26272105
DOI:
10.1007/s12072-015-9658-5
[Indexed for MEDLINE]

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