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Microb Drug Resist. 2016 Mar;22(2):103-8. doi: 10.1089/mdr.2015.0126. Epub 2015 Aug 13.

A Novel 6'-N-Aminoglycoside Acetyltransferase, AAC(6')-Ial, from a Clinical Isolate of Serratia marcescens.

Author information

1
1 Department of Infectious Diseases, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
2
2 Pathogenic Microbe Laboratory, Research Institute , National Center for Global Health and Medicine, Tokyo, Japan .
3
3 Department of Microbiology, Institute of Medicine, Tribhuvan University , Kathmandu, Nepal .
4
4 Department of International Medical-Cooperation, National Center for Global Health and Medicine , Tokyo, Japan .

Abstract

Serratia marcescens IOMTU115 has a novel 6'-N-aminoglycoside acetyltransferase-encoding gene, aac(6')-Ial. The encoded protein AAC(6')-Ial has 146 amino acids, with 91.8% identity to the amino acid sequence of AAC(6')-Ic in S. marcescens SM16 and 97.3% identity to the amino acid sequence of AAC(6')-Iap in S. marcescens WW4. The minimum inhibitory concentrations of aminoglycosides for Escherichia coli expressing AAC(6')-Ial were similar to those for E. coli expressing AAC(6')-Ic or AAC(6')-Iap. Thin-layer chromatography showed that AAC(6')-Ial, AAC(6')-Ic, or AAC(6')-Iap acetylated all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin. Kinetics assays revealed that AAC(6')-Ial is a functional acetyltransferase against aminoglycosides. The aac(6')-Ial gene was located on chromosomal DNA.

PMID:
26270859
DOI:
10.1089/mdr.2015.0126
[Indexed for MEDLINE]

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