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BMC Genomics. 2015 Aug 13;16:603. doi: 10.1186/s12864-015-1794-8.

Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi.

Author information

1
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, Apartado 18, San Juan de Alicante, Alicante, Spain. amartin@umh.es.
2
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, Apartado 18, San Juan de Alicante, Alicante, Spain. lejla.pasic@bf.uni-lj.s.
3
Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia. lejla.pasic@bf.uni-lj.s.
4
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, Apartado 18, San Juan de Alicante, Alicante, Spain. frvalera@umh.es.

Abstract

BACKGROUND:

Haloquadratum walsbyi represents up to 80% of cells in NaCl-saturated brines worldwide, but is notoriously difficult to maintain under laboratory conditions. In order to establish the extent of genetic diversity in a natural population of this microbe, we screened a H. walsbyi enriched metagenomic fosmid library and recovered seven novel version of its cell-wall associated genomic island. The fosmid inserts were sequenced and analysed.

RESULTS:

The novel cell-wall associated islands delineated two major clades within H. walsbyi. The islands predominantly contained genes putatively involved in biosynthesis of surface layer, genes encoding cell surface glycoproteins and genes involved in envelope formation. We further found that these genes are maintained in the population and that the diversity of this region arises through homologous recombination but also through the action of mobile genetic elements, including viruses.

CONCLUSIONS:

The population of H. walsbyi in the studied saltern brine is composed of numerous clonal lineages that differ in surface structures including the cell wall. This type of variation probably reflects a number of mechanisms that minimize the infection rate of predating viruses.

PMID:
26268990
PMCID:
PMC4535781
DOI:
10.1186/s12864-015-1794-8
[Indexed for MEDLINE]
Free PMC Article

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