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J Nat Med. 2016 Jan;70(1):28-35. doi: 10.1007/s11418-015-0933-5. Epub 2015 Aug 13.

Callicarpa longissima extract, carnosol-rich, potently inhibits melanogenesis in B16F10 melanoma cells.

Author information

1
Institute for Molecular Science of Medicine, Aichi Medical University, 1-1, Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
2
Department of Research and Development, Momotani-juntenkan Co. Ltd, 2-4-30, Ichioka, Minato-ku, Osaka, 552-0012, Japan.
3
Tanegashima Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 17007-2, Matsubarayama, Noma, Nakatane-cho, Kumage-gun, Kagoshima, 891-3604, Japan.
4
Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan.
5
Tsukuba Division, Research Center for Medicinal Plant Resources, Japan National Institute of Biomedical Innovation, Health and Nutrition, 1-2, Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
6
Department of Life Science and Biotechnology, Kansai University, Suita, Osaka, 564-8680, Japan.
7
Cell Signaling and Metabolic Disease Laboratory, Department of Fundamental Technological Research, Japan National Institute of Biomedical Innovation, Health and Nutrition, 7-6-8, Asagi-Saito, Ibaraki, Osaka, 567-0085, Japan. mukuharu22@yahoo.co.jp.

Abstract

Cosmetic industries focus on developing materials and resources that regulate skin pigmentation. Melanin, the major pigment in human skin, protects the skin against damage from ultraviolet light. An ethanolic extract of the leaves of Callicarpa longissima inhibits melanin production in B16F10 mouse melanoma cells by suppressing microphthalmia-associated transcription factor (MITF) gene expression. Following purification and analysis using liquid chromatography-mass spectrometry (LC-MS), NMR, and biochemical assays, carnosol was determined to be responsible for the major inhibitory effect of the C. longissima extract on melanin production. Carnosol is an oxidative product of carnosic acid, whose presence in the extract was also confirmed by an authentic reference. The carnosol and carnosic acid content in the extract was approximately 16% (w/w). These results suggest that C. longissima is a novel, useful, and attractive source of skin-whitening agents.

KEYWORDS:

B16F10; Carnosic acid; Carnosol; MITF; Melanogenesis

PMID:
26267810
DOI:
10.1007/s11418-015-0933-5
[Indexed for MEDLINE]

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