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Elife. 2015 Aug 11;4. doi: 10.7554/eLife.05828.

SnRK1-triggered switch of bZIP63 dimerization mediates the low-energy response in plants.

Author information

1
Department of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria.
2
Pharmaceutical Biology, Julius-von-Sachs-Institute, University of Würzburg, Würzburg, Germany.
3
Mass Spectrometry Facility, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
4
Instituto Gulbenkian de Ciência, Oeiras, Portugal.
5
Department of Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany.
6
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Madrid, Spain.
7
Department of Molecular Plant Physiology, Utrecht University, Utrecht, Netherlands.

Abstract

Metabolic adjustment to changing environmental conditions, particularly balancing of growth and defense responses, is crucial for all organisms to survive. The evolutionary conserved AMPK/Snf1/SnRK1 kinases are well-known metabolic master regulators in the low-energy response in animals, yeast and plants. They act at two different levels: by modulating the activity of key metabolic enzymes, and by massive transcriptional reprogramming. While the first part is well established, the latter function is only partially understood in animals and not at all in plants. Here we identified the Arabidopsis transcription factor bZIP63 as key regulator of the starvation response and direct target of the SnRK1 kinase. Phosphorylation of bZIP63 by SnRK1 changed its dimerization preference, thereby affecting target gene expression and ultimately primary metabolism. A bzip63 knock-out mutant exhibited starvation-related phenotypes, which could be functionally complemented by wild type bZIP63, but not by a version harboring point mutations in the identified SnRK1 target sites.

KEYWORDS:

SnRK1 kinase; arabidopsis; bZIP transcription factor; cell biology; metabolic reprogramming; plant biology

PMID:
26263501
PMCID:
PMC4558565
DOI:
10.7554/eLife.05828
[Indexed for MEDLINE]
Free PMC Article

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