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Int J Mol Sci. 2015 Aug 7;16(8):18396-411. doi: 10.3390/ijms160818396.

Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS.

Author information

1
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, Spain. iborras@ugr.es.
2
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento s/n, 18016 Granada, Spain. iborras@ugr.es.
3
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, Spain. mherranz@umh.es.
4
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, Spain. e.barrajon@umh.es.
5
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, Spain. darraez@ugr.es.
6
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento s/n, 18016 Granada, Spain. darraez@ugr.es.
7
Pharmacokinetics and Pharmaceutical Technology Area, Engineering Department, Universidad Miguel Hernández, San Juan de Alicante, 03550 Alicante, Spain. isabel.gonzalez@umh.es.
8
Pharmacokinetics and Pharmaceutical Technology Area, Engineering Department, Universidad Miguel Hernández, San Juan de Alicante, 03550 Alicante, Spain. mbermejo@umh.es.
9
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, Spain. albertof@ugr.es.
10
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento s/n, 18016 Granada, Spain. albertof@ugr.es.
11
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, Spain. vmicol@umh.es.
12
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, Spain. ansegura@ugr.es.
13
Research and Development Functional Food Centre, Health Science Technological Park, Avda Conocimiento s/n, 18016 Granada, Spain. ansegura@ugr.es.

Abstract

Previous findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS), free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine) were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS), and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols.

KEYWORDS:

Caco-2 cells; Hibiscus sabdariffa; UHPLC-ESI-UHR-Qq-TOF-MS; flavonoids; phenolic compounds

PMID:
26262611
PMCID:
PMC4581252
DOI:
10.3390/ijms160818396
[Indexed for MEDLINE]
Free PMC Article

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