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Int J Clin Exp Pathol. 2015 Jun 1;8(6):6169-80. eCollection 2015.

Effects of Polygonatum sibiricum polysaccharide on the osteogenic differentiation of bone mesenchymal stem cells in mice.

Author information

1
Department of Osteopathia, The First Affiliated Hospital of Guangxi Medical University Nanning, Guangxi, China.
2
College of Public Hygiene of Guangxi Medical University Nanning, Guangxi, China.
3
Graduate School of Guangxi Medical University Nanning, Guangxi, China.

Abstract

Polygonatum sibiricum polysaccharide (PSP) is a traditional Chinese medicine and is widely used to treat many diseases for hundreds of years conventionally. This study was to access the effects of PSP on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in the mice. Cells collected from BALB/C mice in the bone marrow were isolated and cultured with osteogenic medium (OM) with different concentrations of PSP. The proliferation and morphological changes of BMSCs were observed using an inverted microscope. Flow cytometric analysis was used to identify the BMSCs. MTT test was performed to analyze the proliferation and viability of the cells. ELISA was used to determine the expression levels of alkaline phosphatase (ALP), osteocalcin (OC), N-terminal propeptide of type I procollagen (PINP) and bone morphogenetic protein-2 (BMP-2). Immunocytochemistry and western blot were respectively used to determine the expressions of bone sialoprotein (BSP) and SPARC/osteonectin (OSN). The growth curves of the proliferation and differentiation of the Control, OM, 17β-E2 and PSP groups were increased. Compared to the Control and OM groups, the expression levels of ALP, OC, PINP and BMP-2 were significantly increased in the PSP induced group (P<0.05). Immunocytochemistry and western blot showed that BSP and SPARC were increased after induction of PSP compared to the OM group (P<0.05). The study demonstrates that PSP promotes the proliferation and enhances the viability of BMSCs during osteogenic differentiation. Therefore, PSP may be a potential treatment of osteoporosis in the clinic.

KEYWORDS:

Polygonatum sibiricum polysaccharide; alkaline phosphatase; bone marrow mesenchymal stem cells; osteoblasts; osteocalcin

PMID:
26261494
PMCID:
PMC4525828
[Indexed for MEDLINE]
Free PMC Article

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