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Inflammation. 2016 Feb;39(1):87-95. doi: 10.1007/s10753-015-0226-x.

Guggulsterone Attenuated Lipopolysaccharide-Induced Inflammatory Responses in Mouse Inner Medullary Collecting Duct-3 Cells.

Kim DG1,2, Bae GS2,3, Jo IJ2,3, Choi SB1, Kim MJ1,2, Jeong JH1,2, Kang DG1,3,4, Lee HS1,3,4, Song HJ1,2, Park SJ5,6,7.

Author information

1
BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, South Korea.
2
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, South Korea.
3
Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749, South Korea.
4
Professional Graduate School of Oriental Medicine and College of Oriental Medicine, Wonkwang University, Shinyong-dong, Iksan, Jeonbuk, 570-749, South Korea.
5
BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, South Korea. parksj08@wku.ac.kr.
6
Department of Herbology, School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 540-749, South Korea. parksj08@wku.ac.kr.
7
Hanbang Body Fluid Research Center, Wonkwang University, Iksan, Jeonbuk, 540-749, South Korea. parksj08@wku.ac.kr.

Abstract

Guggulsterone (GS) is a phytosterol that has been used to treat inflammatory diseases such as colitis, obesity, and thrombosis. Although many previous studies have examined activities of GS, the effect of GS on lipopolysaccharide (LPS)-induced inflammatory responses in mouse inner medullary collecting duct-3 (mIMCD-3) cells have not been examined. Therefore, here, we investigated the anti-inflammatory action of GS on mIMCD-3 cells exposed to LPS. LPS treatment on mIMCD-3 cells produced pro-inflammatory molecules such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) significantly; however, GS treatment significantly inhibited the production of pro-inflammatory molecules. In addition, GS inhibited the degradation of Iκ-Bα and translocation of NF-κB on mIMCD-3 cells. These results suggest that GS could inhibit inflammatory responses in collecting duct cells which could contribute to kidney injury during systemic infection.

KEYWORDS:

guggulsterone (GS); inflammation; inner medullary collecting duct (IMCD); lipopolysaccharide (LPS); toll-like receptor 4 (TLR4)

PMID:
26260258
DOI:
10.1007/s10753-015-0226-x
[Indexed for MEDLINE]

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