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Biotechniques. 2015 Aug 1;59(2):94-8. doi: 10.2144/000114319. eCollection 2015 Aug.

Isolation of segmented filamentous bacteria from complex gut microbiota.

Author information

1
University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MO.
2
MU Metagenomics Center, Columbia, MO.
3
MU Rat Resource and Research Center, Columbia, MO.
4
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO.
5
Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA.

Abstract

Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates.

KEYWORDS:

Candidatus Arthromitus; SFB; commensal; culture; microbiota; segmented filamentous bacteria

PMID:
26260088
DOI:
10.2144/000114319
[Indexed for MEDLINE]

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