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Nat Chem Biol. 2015 Sep;11(9):733-40. doi: 10.1038/nchembio.1885. Epub 2015 Aug 10.

A fungal monooxygenase-derived jasmonate attenuates host innate immunity.

Author information

1
Temasek Life Sciences Laboratory, Singapore.
2
1] Department of Biological Sciences, National University of Singapore, Singapore. [2] Singapore Centre for Environmental Life Sciences Engineering, Singapore. [3] NUS Environmental Research Institute, National University of Singapore, Singapore.
3
1] Temasek Life Sciences Laboratory, Singapore. [2] Department of Biological Sciences, National University of Singapore, Singapore.
4
1] Temasek Life Sciences Laboratory, Singapore. [2] Department of Biological Sciences, National University of Singapore, Singapore. [3] School of Biological Sciences, Nanyang Technological University, Singapore.

Abstract

Distinct modifications fine-tune the activity of jasmonic acid (JA) in regulating plant growth and immunity. Hydroxylated JA (12OH-JA) promotes flower and tuber development but prevents induction of JA signaling, plant defense or both. However, biosynthesis of 12OH-JA has remained elusive. We report here an antibiotic biosynthesis monooxygenase (Abm) that converts endogenous free JA into 12OH-JA in the model rice blast fungus Magnaporthe oryzae. Such fungal 12OH-JA is secreted during host penetration and helps evade the defense response. Loss of Abm in M. oryzae led to accumulation of methyl JA (MeJA), which induces host defense and blocks invasive growth. Exogenously added 12OH-JA markedly attenuated abmΔ-induced immunity in rice. Notably, Abm itself is secreted after invasion and most likely converts plant JA into 12OH-JA to facilitate host colonization. This study sheds light on the chemical arms race during plant-pathogen interaction, reveals Abm as an antifungal target and outlines a synthetic strategy for transformation of a versatile small-molecule phytohormone.

PMID:
26258762
DOI:
10.1038/nchembio.1885
[Indexed for MEDLINE]

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