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Carbohydr Polym. 2015 Nov 5;132:338-44. doi: 10.1016/j.carbpol.2015.06.056. Epub 2015 Jun 30.

Bacillus subtilis 168 levansucrase (SacB) activity affects average levan molecular weight.

Author information

1
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP 62240 Cuernavaca, Morelos, Mexico. Electronic address: jrpd@ibt.unam.mx.
2
Centro de Investigación, DACS-Universidad Juárez Autónoma de Tabasco, Av. Gregorio Méndez no. 2838-A. Col. Tamulte, CP 86150 Villahermosa, Centro, Tabasco, Mexico. Electronic address: angela.avila@ujat.mx.
3
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP 62240 Cuernavaca, Morelos, Mexico; Centro de Investigación, DACS-Universidad Juárez Autónoma de Tabasco, Av. Gregorio Méndez no. 2838-A. Col. Tamulte, CP 86150 Villahermosa, Centro, Tabasco, Mexico.
4
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP 62240 Cuernavaca, Morelos, Mexico. Electronic address: maelena@ibt.unam.mx.
5
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, CP 62240 Cuernavaca, Morelos, Mexico. Electronic address: agustin@ibt.unam.mx.

Abstract

Levan is a fructan polymer that offers a variety of applications in the chemical, health, cosmetic and food industries. Most of the levan applications depend on levan molecular weight, which in turn depends on the source of the synthesizing enzyme and/or on reaction conditions. Here we demonstrate that in the particular case of levansucrase from Bacillus subtilis 168, enzyme concentration is also a factor defining the molecular weight levan distribution. While a bimodal distribution has been reported at the usual enzyme concentrations (1 U/ml equivalent to 0.1 μM levansucrase) we found that a low molecular weight normal distribution is solely obtained al high enzyme concentrations (>5 U/ml equivalent to 0.5 μM levansucrase) while a high normal molecular weight distribution is synthesized at low enzyme doses (0.1 U/ml equivalent to 0.01 μM of levansucrase).

KEYWORDS:

Fructan; Fructosyltransferase; Levan; Levansucrase; SacB; Transferase

PMID:
26256357
DOI:
10.1016/j.carbpol.2015.06.056
[Indexed for MEDLINE]

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