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Chembiochem. 2015 Oct 12;16(15):2244-52. doi: 10.1002/cbic.201500297. Epub 2015 Sep 23.

Changing Biosynthetic Profiles by Expressing bldA in Streptomyces Strains.

Author information

1
Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany.
2
Department of Chemistry, Queen's University, Kingston, ON, K7L 3N6, Canada.
3
Department of Chemistry, Queen's University, Kingston, ON, K7L 3N6, Canada. dlzechel@chem.queensu.ca.
4
Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-Universität Freiburg, Stefan-Meier-Strasse 19, 79104, Freiburg, Germany. andreas.bechthold@pharamzie.uni-freiburg.de.

Abstract

Recently we described an unusual way of activating a cryptic gene cluster when we explored the origin of the bald phenotype of Streptomyces calvus. Complementation of S. calvus with a correct copy of bldA restored sporulation and additionally promoted production of a new natural products. In this study we report on the expression of bldA in several Streptomyces strains that have been described as "poorly sporulating" strains. In seven out of 15 cases, HPLC profiling revealed the production of new compounds, and in two cases the overproduction of known compounds. Two compounds were isolated and their structures were determined.

KEYWORDS:

antibiotics; bldA; gene clusters; gene expression; natural products

PMID:
26255983
DOI:
10.1002/cbic.201500297
[Indexed for MEDLINE]

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