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Proteomics Clin Appl. 2016 Feb;10(2):144-55. doi: 10.1002/prca.201400204. Epub 2015 Nov 19.

18O proteomics reveal increased human apolipoprotein CIII in Hispanic HIV-1+ women with HAART that use cocaine.

Author information

1
Department of Microbiology, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico.
2
Laboratorio de Proteómica Cardiovascular, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
3
Department of Biochemistry, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico.
4
Department of Biostatistics and Epidemiology, Graduate School of Public Health, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico.
5
Department of Physiology, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico.
6
Department of Internal Medicine, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico.

Abstract

PURPOSE:

Drug abuse is a major risk factor in the development and progression of HIV-1. This study defines the alterations in the plasma proteome of HIV-1-infected women that use cocaine.

EXPERIMENTAL DESIGN:

Plasma samples from 12 HIV-seropositive Hispanic women under antiretroviral therapy were selected for this study. Six sample pairs were matched between nondrug users and cocaine users. After IgG and albumin depletion, SDS-PAGE, and in-gel digestion, peptides from nondrug users and cocaine users were labeled with (16) O and (18) O, respectively, and subjected to LC-MS/MS and quantitation using Proteome Discover and QuiXoT softwares and validated by ELISA.

RESULTS:

A total of 1015 proteins were identified at 1% false discovery rates (FDR). Statistical analyses revealed 13 proteins with significant changes between the two groups, cocaine and noncocaine users (p < 0.05). The great majority pertained to protection defense function and the rest pertained to transport, homeostatic, regulation, and binding of ligands. Apolipoprotein CIII was increased in plasma of HIV+ Hispanic women positive for cocaine compared to HIV+ nondrug users (p ≤ 0.05).

CONCLUSIONS AND CLINICAL RELEVANCE:

Increased human apolipoprotein CIII warrants that these patients be carefully monitored to avoid the increased risk of cardiovascular events associated with HIV, HAART, and cocaine use.

KEYWORDS:

18O labeling; Apolipoprotein CIII; Cocaine; HIV; Quantitative proteomics

PMID:
26255783
PMCID:
PMC4977997
DOI:
10.1002/prca.201400204
[Indexed for MEDLINE]
Free PMC Article

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