Send to

Choose Destination
Insect Biochem Mol Biol. 2015 Oct;65:47-56. doi: 10.1016/j.ibmb.2015.08.002. Epub 2015 Aug 7.

Transcription factors, CncC and Maf, regulate expression of CYP6BQ genes responsible for deltamethrin resistance in Tribolium castaneum.

Author information

Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA.
Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA. Electronic address:


Insecticide resistance is a global problem that presents an ongoing challenge to control insects that destroy crops, trees and transmit diseases. Dramatic progress has been made during the last decade on identification of insecticide resistance-associated genes. In one of the most common resistance mechanisms, insects acquire resistance by increasing the levels of their detoxification enzymes especially the cytochrome P450 monooxygenases (P450's). Previous studies in our laboratory showed that the pyrethroid resistance in QTC279 strain of Tribolium castaneum is achieved through constitutive overexpression of the P450 gene CYP6BQ9 by 200-fold higher in the resistant strain as compared to that in the susceptible strain. RNAi-aided knockdown in the expression of probable genes that regulate P450 gene expression in QTC279 identified cap 'n' collar C (CncC) and muscle aponeurosis fibromatosis (Maf) family transcription factors as the key regulator of these genes, CncC and Maf regulate expression of multiple genes in the CYP6BQ cluster. Studies on the promoters of these genes using reporter assays identified binding sites that mediate CncC and Maf regulation of CYP6BQ gene expression.


Insecticide resistance; P450; Tribolium; Xenobiotic transcription factors

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center