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J Microbiol Methods. 2015 Oct;117:136-8. doi: 10.1016/j.mimet.2015.08.001. Epub 2015 Aug 5.

High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.

Author information

1
Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.
2
Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy. Electronic address: f.campanile@unict.it.

Abstract

We report an implemented molecular-typing-method based on HRMA to detect SNPs within MLST loci, characterizing 100 clinical MRSA and 11 control strains, representative of Italian clones. The results provide solid evidence that HRMT could be a fast, cost-effective and reliable alternative to MLST, for MRSA molecular epidemiology.

KEYWORDS:

Cost and time reduction; High Resolution Melting Typing (HRMT); Methicillin-resistant Staphylococcus aureus (MRSA); Molecular-epidemiology; Single-nucleotide polymorphisms (SNPs)

PMID:
26255133
DOI:
10.1016/j.mimet.2015.08.001
[Indexed for MEDLINE]

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