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Anticancer Res. 2015 Sep;35(9):4765-75.

Chemoprevention of Azoxymethane-induced Colonic Carcinogenesis in Balb/c mice Using a Modified Pectin Alginate Probiotic.

Author information

1
Department of Biotechnology and Food Technology, Durban University of Technology, Steve Biko, KwaZulu-Natal, South Africa.
2
Optics and Image Centre, Doris Duke Medical Research Institute, College of Health Sciences, University of KwaZulu-Natal, KwaZulu-Natal, South Africa.
3
Faculty of Applied Science, Cape Peninsula University of Technology, Cape Town, South Africa reddyl@cput.ac.za lalinisai@gmail.com.

Abstract

BACKGROUND:

Increased intake of probiotic dietary fibre reduces colonic cancer risk. Modified citrus pectin (MCP) requires optimal bioactivity to inhibit galectin-3 (GAL-3) and vascular endothelial growth factor (VEGF). This study evaluated the preventative effect of modified pectin alginate (MCPA) probiotic microbeads on azoxymethane (AOM)-induced colonic carcinogenesis in Balb/c mice.

MATERIALS AND METHODS:

Optimization of AOM dose duration: 10-15 mg/kg was administered for 2-4 weeks. The optimal AOM dose was initiated prior to intake of MCPA, alginate probiotic (AP) microbeads and MCP in Balb/c mice for 16 weeks; samples were analyzed for colonic histopathology and immunohistochemistry.

RESULTS:

AOM at 15 mg/kg for 4 weeks induced optimal GAL-3 and VEGF immunostaining. Furthermore, MCPA treatment reduced GAL-3 expression in the colon of AOM-treated mice compared to MCP.

CONCLUSION:

MCPA probiotic microbeads increase bioactivity and chemopreventative effect against pre-cancerous colonic lesions and adenocarcinoma through inhibition of GAL-3 and VEGF in the Balb/c mouse model of colonic carcinogenesis.

KEYWORDS:

Azoxymethane; colonic cancer; galectin-3; immunohistochemistry; modified citrus pectin; probiotic

PMID:
26254367
[Indexed for MEDLINE]

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