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Nanomedicine (Lond). 2015;10(15):2339-48. doi: 10.2217/NNM.15.76. Epub 2015 Aug 7.

PLGA-encapsulated perfluorocarbon nanoparticles for simultaneous visualization of distinct cell populations by 19F MRI.

Author information

1
Department of Tumor Immunology, & Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands.
2
Instituto de FĂ­sica del Litoral (CONICET - UNL), Santa Fe, Argentina.
3
Molecular Imaging, Leiden University Medical Center, Leiden, The Netherlands.
4
Department of Radiology, Radboud University Medical Center, Nijmegen, The Netherlands.
5
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.

Abstract

AIM:

In vivo imaging using (19)F MRI is advantageous, due to its ability to quantify cell numbers, but is limited for a lack of suitable labels. Here, we formulate two stable and clinically applicable labels for tracking two populations of primary human dendritic cells (DCs) simultaneously.

MATERIALS & METHODS:

Plasmacytoid and myeloid DCs are able to take up sufficient nanoparticles (200 nm) for imaging (10(12 19)F's per cell), despite being relatively nonphagocytic.

RESULTS:

Clinically relevant numbers of labeled DCs could be imaged in about 10 min, even on a clinical scanner.

CONCLUSION:

We demonstrate the use of perfluorocarbon nanoparticles for simultaneous (19)F MRI of distinct cell populations in a clinical setting, without spectroscopic imaging.

KEYWORDS:

19F MRI; PLGA; cell therapy; fluorescence; in vivo imaging; perfluorocarbons

PMID:
26251876
DOI:
10.2217/NNM.15.76
[Indexed for MEDLINE]

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