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J Shoulder Elbow Surg. 2015 Nov;24(11):1764-73. doi: 10.1016/j.jse.2015.06.009. Epub 2015 Aug 4.

Evaluation of a collagen-coated, resorbable fiber scaffold loaded with a peptide basic fibroblast growth factor mimetic in a sheep model of rotator cuff repair.

Author information

1
DRP Biomedical, Carlsbad, CA, USA. Electronic address: 2dalesmail@gmail.com.
2
Microvascular Tissues, Boston, MA, USA.
3
Advanced Biological Concepts, Los Alamitos, CA, USA.
4
Golden Orthopedic, Delray Beach, FL, USA.
5
New West Sports Medicine, Kearney, NE, USA.
6
Miami Sports Medicine, Miami, FL, USA.
7
SimonMed Imaging, Scottsdale, AZ, USA.
8
Experimental Pathology Laboratories, Sterling, VA, USA.
9
Charter Preclinical Services, Hudson, MA, USA.
10
Union Memorial Hospital, Baltimore, MD, USA.

Abstract

BACKGROUND:

A new scaffold design combined with a peptide growth factor was tested prospectively for safety and for improved tendon healing in sheep.

METHODS:

The infraspinatus tendon was detached and then surgically repaired to the humerus using sutures and anchors in 50 adult sheep. The repairs in 40 of these sheep were reinforced with a scaffold containing F2A, a peptide mimetic of basic fibroblast growth factor. The sheep were examined after 8 or 26 weeks with magnetic resonance imaging, full necropsy, and histopathologic analysis. A second cohort of 30 sheep underwent surgical repair--20 with scaffolds containing F2A. The 30 shoulders were tested mechanically after 8 weeks.

RESULTS:

The scaffold and F2A showed no toxicity. Scaffold-repaired tendons were 31% thicker than surgically repaired controls (P = .037) at 8 weeks. There was more new bone formed at the tendon footprint in sheep treated with F2A. Surgically repaired tendons delaminated from the humerus across 14% of the footprint area. The extent of delamination decreased to 1.3% with increasing doses of F2A (P = .004). More of the repair tissue at the footprint was tendon-like in the peptide-treated sheep. On mechanical testing, only 7 shoulders tore at the repair site. The repairs in the other 23 shoulders were already stronger than the midsubstance tendon at 8 weeks.

CONCLUSIONS:

The new scaffold and peptide safely improved tendon healing.

KEYWORDS:

Scaffold; bFGF; mimetic; peptide; poly-4-hydroxybutyrate; rotator cuff; sheep

PMID:
26251198
DOI:
10.1016/j.jse.2015.06.009
[Indexed for MEDLINE]

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