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Mater Sci Eng C Mater Biol Appl. 2015 Nov 1;56:451-6. doi: 10.1016/j.msec.2015.07.007. Epub 2015 Jul 15.

Porcine kidneys as a source of ECM scaffold for kidney regeneration.

Author information

1
Department of Urology, The Second Hospital, Shandong University, China.
2
Department of Kidney Transplantation, The Second Hospital, Shandong University, China.
3
Department of Central Research Laboratory, The Second Hospital, Shandong University, China.
4
Department of Urology, The Second Hospital, Shandong University, China. Electronic address: zhaoshengtian@sdu.edu.cn.

Abstract

OBJECTIVE:

To produce and examine decellularized kidney scaffolds from porcine as a platform for kidney regeneration research.

METHODS:

Porcine kidneys were decellularized with sodium dodecyl sulfate solution and Triton X-100 after the blood was rinsed. Then the renal ECM scaffolds were examined for vascular imaging, histology to investigate the vascular patency, degree of decellularization.

RESULTS:

Renal ECM scaffolds of porcine kidneys were successfully produced. Decellularized renal scaffolds retained intact microarchitecture including the renal vasculature and essential extracellular matrix components.

CONCLUSION:

We have developed an excellent decellularization method that can be used in large organs. These scaffolds maintain their basic components, and show intact vasculature system. This represents a step toward development of a transplantable organ using tissue engineering techniques.

KEYWORDS:

Acellular scaffolds; Decellularization; Kidney regeneration; Whole organ engineering

PMID:
26249614
DOI:
10.1016/j.msec.2015.07.007
[Indexed for MEDLINE]

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