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Mater Sci Eng C Mater Biol Appl. 2015 Nov 1;56:141-53. doi: 10.1016/j.msec.2015.06.004. Epub 2015 Jun 9.

Composite ECM-alginate microfibers produced by microfluidics as scaffolds with biomineralization potential.

Author information

1
Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, Italy.
2
Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
3
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

Abstract

A novel approach to produce artificial bone composites (microfibers) with distinctive features mimicking natural tissue was investigated. Currently proposed inorganic materials (e.g. apatite matrixes) lack self-assembly and thereby limit interactions between cells and the material. The present work investigates the feasibility of creating "bio-inspired materials" specifically designed to overcome certain limitations inherent to current biomaterials. We examined the dimensions, morphology, and constitutive features of a composite hydrogel which combined an alginate based microfiber with a gelatin solution or a particulate form of urinary bladder matrix (UBM). The effectiveness of the composite microfibers to induce and modulate osteoblastic differentiation in three-dimensional (3D) scaffolds without altering the viability and morphological characteristics of the cells was investigated. The present study describes a novel alginate microfiber production method with the use of microfluidics. The microfluidic procedure allowed for precise tuning of microfibers which resulted in enhanced viability and function of embedded cells.

KEYWORDS:

Alginate; Microfibers; Microfluidics; Osteoblastic differentiation; UBM

PMID:
26249575
DOI:
10.1016/j.msec.2015.06.004
[Indexed for MEDLINE]

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