Format

Send to

Choose Destination
J Appl Microbiol. 2015 Oct;119(4):1127-37. doi: 10.1111/jam.12923.

Bifidobacterium longum BBMN68-specific modulated dendritic cells alleviate allergic responses to bovine β-lactoglobulin in mice.

Yang J1,2,3, Zhang H1,2,3, Jiang L1,2,3, Guo H1,2,3, Luo X4, Ren F1,2,3.

Author information

1
The Innovation Centre of Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
2
Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
3
Beijing Laboratory of Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
4
Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Abstract

AIMS:

This study was designed to demonstrate the protective effects of Bifidobacterium longum BBMN68-specific modulated dendritic cells (DCs) on allergic inflammation in β-lactoglobulin (BLG)-sensitized mice.

METHODS AND RESULTS:

BALB/c mice were sensitized to BLG in accordance with a model of food allergy protocol and given oral BBMN68 daily. BBMN68 was found to significantly reduce BLG-specific hypersensitivity reactions by suppressing the aberrant balance of Th1/Th2 responses with increasing the number of CD4+CD25+Foxp3+ Treg cells in mesenteric lymph nodes (MLN) by 48·1%. The level of CD103+DCs was up-regulated by 136·7 and 56·2% in payer's patches and MLN, respectively, in response to the lower expression levels of cell-surface molecules (CD86 and MHC-II) induced by BBMN68 supplementation. The CD11c+DCs isolated from BBMN68 mice showed 45·6% more Foxp3+ expression in vitro.

CONCLUSIONS:

These data suggest that BBMN68-specific induction of CD11c+CD103+DCs and semi-mature DCs reduce BLG allergic reactions.

SIGNIFICANCE AND IMPACT OF THE STUDY:

These data confirm that BBMN68 may be a suitable therapeutic approach to the alleviation of food allergies, and BBMN68-specific induction of CD11c+CD103+DCs and semi-mature DCs are associated with this protection.

KEYWORDS:

Bifidobacterium longum BBMN68; T regulatory cell; cow milk allergy; dendritic cell; oral tolerance

PMID:
26248977
DOI:
10.1111/jam.12923
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center