Format

Send to

Choose Destination
Sci Rep. 2015 Aug 7;5:12985. doi: 10.1038/srep12985.

Basal Autophagy Is Required for Herpes simplex Virus-2 Infection.

Author information

1
1] Department of Microbiology and Immunology, University of Illinois, Chicago, IL USA, 60612 [2] Department of Ophthalmology and Visual Sciences, University of Illinois Medical Center, Chicago, IL USA, 60612.

Abstract

Autophagy is a conserved catabolic process of the cell, which plays an important role in regulating plethora of infections. The role of autophagy in Herpes simplex virus-2 (HSV-2) infection is unknown. Here, we found that HSV-2 does not allow induction of an autophagic response to infection, but maintains basal autophagy levels mostly unchanged during productive infection. Thus, we investigated the importance of basal autophagy for HSV-2 infection, using pharmacological autophagy suppression or cells genetically deficient in an autophagy-essential gene (ATG5). Interference with basal autophagy flux in cells significantly reduced viral replication and diminished the infection. These results indicate that basal autophagy plays an indispensable role required for a productive infection. Importantly, this study draws a sharp distinction between induced and basal autophagy, where the former acts as a viral clearance mechanism abrogating infection, while the latter supports infection.

PMID:
26248741
PMCID:
PMC4528227
DOI:
10.1038/srep12985
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center