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Evid Based Complement Alternat Med. 2015;2015:502454. doi: 10.1155/2015/502454. Epub 2015 Jul 13.

Preparation, Characterization, and Pharmacological Activity of Cymbopogon winterianus Jowitt ex Bor (Poaceae) Leaf Essential Oil of β-Cyclodextrin Inclusion Complexes.

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Postgraduate Program in Health Science, Federal University of Sergipe, 49060-100 Aracaju, SE, Brazil.
Department of Chemistry, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
Department of Morphology, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
Department of Agronomy, Federal University of Sergipe, 49100-000 São Cristóvão, SE, Brazil.
Postgraduate Program in Natural Products and Bioactive Synthetics (PgPNSB), Federal University of Paraíba, 58051-900 João Pessoa, PB, Brazil.


This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in β-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in β-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50-200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamate-induced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p < 0.05) reduced. The number of Fos-positive cells was significantly changed in the dorsal raphe nucleus (p < 0.01), locus coeruleus (p < 0.001), trigeminal nucleus (p < 0.05), and trigeminal thalamic tract (p < 0.05). LEO-CD did not cause changes in motor coordination in the rota-rod test. Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.

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