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Vaccine. 2015 Sep 11;33(38):4827-36. doi: 10.1016/j.vaccine.2015.07.072. Epub 2015 Aug 1.

Development of oral CTL vaccine using a CTP-integrated Sabin 1 poliovirus-based vector system.

Author information

1
Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea.
2
Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Gyeonggi-Do, Republic of Korea. Electronic address: ysbae04@skku.edu.

Abstract

We developed a CTL vaccine vector by modification of the RPS-Vax system, a mucosal vaccine vector derived from a poliovirus Sabin 1 strain, and generated an oral CTL vaccine against HIV-1. A DNA fragment encoding a cytoplasmic transduction peptide (CTP) was integrated into the RPS-Vax system to generate RPS-CTP, a CTL vaccine vector. An HIV-1 p24 cDNA fragment was introduced into the RPS-CTP vector system and a recombinant poliovirus (rec-PV) named vRPS-CTP/p24 was produced. vRPS-CTP/p24 was genetically stable and efficiently induced Th1 immunity and p24-specific CTLs in immunized poliovirus receptor-transgenic (PVR-Tg) mice. In challenge experiments, PVR-Tg mice that were pre-immunized orally with vRPS-CTP/p24 were resistant to challenge with a lethal dose of p24-expressing recombinant vaccinia virus (rMVA-p24). These results suggested that the RPS-CTP vector system had potential for developing oral CTL vaccines against infectious diseases.

KEYWORDS:

HIV-1 p24; Oral CTL vaccine; Poliovirus Sabin 1 vector; RPS-CTP CTL vector; Recombinant poliovirus; p24-specific CTL

PMID:
26241946
DOI:
10.1016/j.vaccine.2015.07.072
[Indexed for MEDLINE]

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