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Cold Spring Harb Protoc. 2015 Aug 3;2015(8):711-4. doi: 10.1101/pdb.top077503.

Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, Washington 98195;
2
Department of Genome Sciences, University of Washington, Seattle, Washington 98195; Department of Medicine, University of Washington, Seattle, Washington 98195; Howard Hughes Medical Institute, Seattle, Washington 98195.

Abstract

Deep mutational scanning is a method that makes use of next-generation sequencing technology to measure in a single experiment the activity of 10(5) or more unique variants of a protein. Because of this depth of mutational coverage, this strategy provides data that can be analyzed to reveal many protein properties. Deep mutational scanning approaches are particularly amenable to being performed in Saccharomyces cerevisiae, given the extensive toolkit of reagents and technologies available for this organism.

PMID:
26240414
DOI:
10.1101/pdb.top077503
[Indexed for MEDLINE]

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